immunoculttm human th2 differentiation supplement Search Results


immunocult human th2 differentiation supplement  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc immunocult human th2 differentiation supplement
Serpinb10-knockdown mice had diminished numbers of <t>Th2</t> cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
Immunocult Human Th2 Differentiation Supplement, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immunocult human th2 differentiation supplement/product/STEMCELL Technologies Inc
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immunocult human th2 differentiation supplement - by Bioz Stars, 2026-03
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immunoculttm human th2 differentiation supplement  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc immunoculttm human th2 differentiation supplement
Serpinb10-knockdown mice had diminished numbers of <t>Th2</t> cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
Immunoculttm Human Th2 Differentiation Supplement, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immunoculttm human th2 differentiation supplement/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
immunoculttm human th2 differentiation supplement - by Bioz Stars, 2026-03
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ril-4  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc ril-4
Serpinb10-knockdown mice had diminished numbers of <t>Th2</t> cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
Ril 4, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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differentiation supplement cocktail for t regulatory cells (treg)  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc differentiation supplement cocktail for t regulatory cells (treg)
Serpinb10-knockdown mice had diminished numbers of <t>Th2</t> cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
Differentiation Supplement Cocktail For T Regulatory Cells (Treg), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunoculttm human cd3/cd28/cd2 t cell activator  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc immunoculttm human cd3/cd28/cd2 t cell activator
Serpinb10-knockdown mice had diminished numbers of <t>Th2</t> cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
Immunoculttm Human Cd3/Cd28/Cd2 T Cell Activator, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunocult human th1 differentiation supplement  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc immunocult human th1 differentiation supplement
Serpinb10-knockdown mice had diminished numbers of <t>Th2</t> cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
Immunocult Human Th1 Differentiation Supplement, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t-cell expansion medium  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc t-cell expansion medium
Serpinb10-knockdown mice had diminished numbers of <t>Th2</t> cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
T Cell Expansion Medium, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il21  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc il21
Serpinb10-knockdown mice had diminished numbers of <t>Th2</t> cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
Il21, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-ifnγ neutralizing antibodies  (Bio X Cell)
90
Bio X Cell anti-ifnγ neutralizing antibodies
Serpinb10-knockdown mice had diminished numbers of <t>Th2</t> cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
Anti Ifnγ Neutralizing Antibodies, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human interleukin-4  (PeproTech)
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PeproTech recombinant human interleukin-4
Serpinb10-knockdown mice had diminished numbers of <t>Th2</t> cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
Recombinant Human Interleukin 4, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cryopreserved human cd4 t cells stem cell  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc cryopreserved human cd4 t cells stem cell
Serpinb10-knockdown mice had diminished numbers of <t>Th2</t> cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
Cryopreserved Human Cd4 T Cells Stem Cell, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Serpinb10-knockdown mice had diminished numbers of Th2 cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.

Journal: Respiratory Research

Article Title: SERPINB10 contributes to asthma by inhibiting the apoptosis of allergenic Th2 cells

doi: 10.1186/s12931-021-01757-1

Figure Lengend Snippet: Serpinb10-knockdown mice had diminished numbers of Th2 cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.

Article Snippet: ImmunoCult Human Th1 Differentiation Supplement (StemCell Technologies) and ImmunoCult Human Th2 Differentiation Supplement (StemCell Technologies) were added to direct the differentiation of Th1 and Th2 cells, respectively.

Techniques: Knockdown

Expression level of SERPINB10 in human Th cells. a flow cytometric plots for Th1 or Th2 polarized CD4 T cells from patients with asthma after intracellular staining for IFN-γ or IL-4. b the transcript level of IFNG, T-bet, IL-4 and GATA-3 in polarized Th1 and Th2 cells was measured using quantitative PCR. c cytokine levels in culture supernatant of polarized Th1 or Th2 cells were measured by LEGENDplex human Th cytokine panel. d SERPINB10 transcript level (fold change over naïve CD4 T cells) in Th1 or Th2 cells after no stimulation or stimulation with anti-CD3 or cytokine (IFN-γ for Th1 and IL-4 for Th2). n = 16 patient samples. Data were means ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant

Journal: Respiratory Research

Article Title: SERPINB10 contributes to asthma by inhibiting the apoptosis of allergenic Th2 cells

doi: 10.1186/s12931-021-01757-1

Figure Lengend Snippet: Expression level of SERPINB10 in human Th cells. a flow cytometric plots for Th1 or Th2 polarized CD4 T cells from patients with asthma after intracellular staining for IFN-γ or IL-4. b the transcript level of IFNG, T-bet, IL-4 and GATA-3 in polarized Th1 and Th2 cells was measured using quantitative PCR. c cytokine levels in culture supernatant of polarized Th1 or Th2 cells were measured by LEGENDplex human Th cytokine panel. d SERPINB10 transcript level (fold change over naïve CD4 T cells) in Th1 or Th2 cells after no stimulation or stimulation with anti-CD3 or cytokine (IFN-γ for Th1 and IL-4 for Th2). n = 16 patient samples. Data were means ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant

Article Snippet: ImmunoCult Human Th1 Differentiation Supplement (StemCell Technologies) and ImmunoCult Human Th2 Differentiation Supplement (StemCell Technologies) were added to direct the differentiation of Th1 and Th2 cells, respectively.

Techniques: Expressing, Staining, Real-time Polymerase Chain Reaction

Knockdown of SERPINB10 expression affects survival of Th2 cells. a representative light micrograph of lentivirus (LV)-transduced CD4 T cells (top and bottom panels are pictures of the same cells in fluorescence mode and normal light mode respectively). b and c flow cytometric data for green fluorescent protein (GFP) + percentage of lentivirus-transduced CD4 T cells. d SERPINB10 transcript level in lentivirus-transduced CD4 T cells (fold change compared with ACTB housekeeping gene). e percentage of IFN-γ + or IL-4 + polarized CD4 cells upon completion of Th1 and Th2 polarization. f percentage of IFN-γ + or IL-4 + polarized CD4 cells after completion of polarization and rested for another 8 days. g flow cytometric histogram for negative control (solid histogram) or SERPINB10-knockdown (line) Th1 and Th2 cells after staining with AnnexinV. Percentage of AnnexinV-positive cells were shown in histograms. Data were pooled from three independent experiment; n = 4 wells per group for each independent experiment. Data were mean ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant

Journal: Respiratory Research

Article Title: SERPINB10 contributes to asthma by inhibiting the apoptosis of allergenic Th2 cells

doi: 10.1186/s12931-021-01757-1

Figure Lengend Snippet: Knockdown of SERPINB10 expression affects survival of Th2 cells. a representative light micrograph of lentivirus (LV)-transduced CD4 T cells (top and bottom panels are pictures of the same cells in fluorescence mode and normal light mode respectively). b and c flow cytometric data for green fluorescent protein (GFP) + percentage of lentivirus-transduced CD4 T cells. d SERPINB10 transcript level in lentivirus-transduced CD4 T cells (fold change compared with ACTB housekeeping gene). e percentage of IFN-γ + or IL-4 + polarized CD4 cells upon completion of Th1 and Th2 polarization. f percentage of IFN-γ + or IL-4 + polarized CD4 cells after completion of polarization and rested for another 8 days. g flow cytometric histogram for negative control (solid histogram) or SERPINB10-knockdown (line) Th1 and Th2 cells after staining with AnnexinV. Percentage of AnnexinV-positive cells were shown in histograms. Data were pooled from three independent experiment; n = 4 wells per group for each independent experiment. Data were mean ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant

Article Snippet: ImmunoCult Human Th1 Differentiation Supplement (StemCell Technologies) and ImmunoCult Human Th2 Differentiation Supplement (StemCell Technologies) were added to direct the differentiation of Th1 and Th2 cells, respectively.

Techniques: Knockdown, Expressing, Fluorescence, Negative Control, Staining